AGO1 antibody, rabbit polyclonal
Background
Encodes an RNA Slicer that selectively recruits microRNAs and siRNAs. There is currently no evidence that
AGO1 Slicer is in a high molecular weight RNA-induced silencing complex (RISC). Mutants are defective in
post-transcriptional gene silencing and have pleiotropic developmental and morphological defects. Through its
action on the regulation of ARF17 expression, the protein regulates genes involved at the cross talk between auxin
and light signaling during adventitious root development. AGO1 seems to be targeted for degradation by silencing
suppressor F-box-containing proteins from Turnip yellow virus and Cucurbit aphid-borne yellow virus.
Other names: AGO1, ARGONAUTE 1, ATAGO1, ICU9
Immunogen: Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein
containing the C-terminal region of Arabidopsis thaliana AGO1 (AT1G48410). Antibodies were purified by
affinity purification using immunogen.
Concentration: 1.0mg/ml
Tested applications: Western blot (WB), IF
Expected/apparent MW: 116/130 kDa
Species specificity: Arabidopsis thaliana
Recommended dilution: 1 : 500-2000 (WB, detect endogenous protein ), 1: 100 - 1: 500 (IF)(optimal dilutions
should be determined by the end use.)
Storage Buffer: PBS Buffer, pH7.4
Storage: Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Stable for 2
years from date of receipt. Aliquot to avoid repeated freezing and thawing.
