DCL1 antibody, rabbit polyclonal
Background
Encodes a Dicer homolog. Dicer is a RNA helicase involved in microRNA processing. Mutations in this locus can
result in embryo lethality. Embryo shape at seed maturity is globular-elongate. Other mutants convert the floral
meristems to an indeterminate state, others yet show defects in ovule development. mRNA is expressed in all shoot
tissues. DCL1 is able to produce miRNAs and siRNAs. The mRNA is cell-to-cell mobile.
Other names: ABNORMAL SUSPENSOR 1, ASU1, ATDCL1, CAF, CARPEL FACTORY, DCL1, DICER-LIKE
1, EMB60, EMB76, EMBRYO DEFECTIVE 60, EMBRYO DEFECTIVE 76, SHORT INTEGUMENTS 1, SIN1,
SUS1, SUSPENSOR 1
Immunogen: KLH-conugated synthetic peptide, chosen from Arabidopsis thaliana DCL1 protein sequence
AT1G01040.
Concentration: 1.0mg/ml
Expected/apparent MW: 213 kDa
Tested applications: Western blot (WB), IF
Species specificity: Arabidopsis thaliana
Recommended dilution: 1 : 500-2000 (WB, detect endogenous protein ), 1: 100 - 1: 500 (IF)(optimal dilutions
should be determined by the end use.)
Storage Buffer: PBS Buffer, pH7.4
Storage: Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Stable for 2
years from date of receipt. Aliquot to avoid repeated freezing and thawing.
* Please note: FOR RESEARCH USE ONLY
