COP1 antibody, rabbit polyclonal
Background
Constitutive photomorphogenesis protein 1 (COP1) is an E3 ubiquitin-protein ligase that acts as a repressor of
photomorphogenesis and as an activator of etiolation in darkness. E3 ubiquitin ligases accept ubiquitin from an E2
ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted
substrates. COP1 represses photomorphogenesis in darkness by mediating ubiquitination and subsequent
proteasomal degradation of light-induced transcription factors such as HY5, HYH and LAF1. Cop1 also
down-regulates MYB21, probably via ubiquitination process. Light stimuli abrogate the repression of
photomorphogenesis, possibly due to its localization to the cytoplasm. COP1 could play a role in switching
between skotomorphogenetic and photomorphogenetic pathways. Represses photomorphogenesis and induces
skotomorphogenesis in the dark. Contains a ring finger zinc-binding motif, a coiled-coil domain, and several
WD-40 repeats, similar to G-beta proteins. The C-terminus has homology to TAFII80, a subunit of the TFIID
component of the RNA polymerase II of Drosophila. Nuclear localization in the dark and cytoplasmic in the light.
The mRNA is cell-to-cell mobile.
Other names:
ARABIDOPSIS THALIANA CONSTITUTIVE PHOTOMORPHOGENIC 1, ATCOP1, CONSTITUTIVE
PHOTOMORPHOGENIC 1, COP1, DEETIOLATED MUTANT 340, DET340, EMB168, EMBRYO
DEFECTIVE 168, FUS1, FUSCA 1 AT2G32950
Immunogen: Recombinant protein
Concentration: 1.0mg/ml
Tested applications: Western blot (WB), IF
Expected/apparent MW: 76/82 kDa
Species specificity: Arabidopsis thaliana
Recommended dilution: 1 : 500-2000 (WB, detect endogenous protein ), 1: 100 - 1: 500(optimal dilutions should
be determined by the end use.)
Storage Buffer: PBS Buffer, pH7.4
Storage: Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Stable for 2
years from date of receipt. Aliquot to avoid repeated freezing and thawing.
