ARF1 antibody, rabbit polyclonal
Background
The ARF1 (ADP-ribosylation factor 1) protein is localized to the Golgi apparatus and has a central role in
intra-Golgi transport. It is a small GTPase that undergoes a GDP/GTP nucleotide exchange cycle and it is an
important regulator of cellular trafficking. Gene encoding ADP-ribosylation factor and similar to other ARFs and
ARF-like proteins. A member of ARF GTPase family. Arabidopsis has 21 known members, known to be essential
for vesicle coating and uncoating and functions in GTP-binding. The gene is shown to play a role in cell division,
cell expansion and cellulose production using antisense construct.
Other names: ADP-RIBOSYLATION FACTOR, ADP-RIBOSYLATION FACTOR 1, ADP-RIBOSYLATION
FACTOR 1A, ARF1, ATARF, ATARF1, ATARFA1AAT1G23490
Immunogen: Rabbit polyclonal antibodies were produced by immunizing animals with a recombinant GST fusion
of full length of Arabidopsis thaliana ARF1 (AT2G47170). Antibodies were purified by affinity purification using
immunogen.
Concentration: 1.0mg/ml
Tested applications: Western blot (WB), IF
Expected/apparent MW: 21 kDa
Species specificity: Arabidopsis thaliana
Recommended dilution: 1 : 500-2000 (WB, detect endogenous protein ), 1: 100 - 1: 500 (IF)(optimal dilutions
should be determined by the end use.)
Storage Buffer: PBS Buffer, pH7.4
Storage: Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Stable for 2
years from date of receipt. Aliquot to avoid repeated freezing and thawing.
* Please note: FOR RESEARCH USE ONLY
Fig. 1. Western blot analysis of total protein extracts from wild type Arabidopsis seedlings, using anti-ARF1 at RT for 1 hour.
