Anti-Amanitin monoclonal antibody

Anti- α-Amanitin antibody, mAb

Background

α-Amanitin is the principal toxin of several deadly poisonous mushrooms, exerting its toxic function by

inhibiting RNA-polymerase II. α-Amanitin decreases TAF15 mRNA and TAF15 protein levels in MKN45 cells,

and inhibits the RNAPII activity towards TAF15 mRNA. α-Amanitin dramatically decreases the expression of gap

junctional genes (Gja1, Gja4 and Gjc1) and FSHr and LHr in cumulus cells.

Immunogen: α-Amanitin-KLH

Specificity: Specifically recognizes the target-α-Amanitin

Source: Mouse, Monoclonal Clone #02

Isotype: IgG1

Conjugate: Unconjugated

Concentration: 1.0 mg/ml

Purity: > 90% as determined by SDS-PAGE

Tested applications: ELISA

Recommended dilution: 1:1,000 - 1:5,000 (optimal dilutions should be determined by the end use.)

Storage Buffer: PBS Buffer, pH7.4

Storage: Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Stable for 2

years from date of receipt. Aliquot to avoid repeated freezing and thawing.

* Please note: FOR RESEARCH USE ONLY